Overview

  • Product nameAnti-Anillin antibody
    See all Anillin primary antibodies
  • Description
    Rabbit polyclonal to Anillin
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human Anillin (NP_061155.2)

  • Positive control
    • HeLa and 293T whole cell lysates This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
IP Use a concentration of 2 - 5 µg/ml.

Target

  • FunctionRequired for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
  • Tissue specificityUbiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
  • Sequence similaritiesContains 1 PH domain.
  • Developmental stageExpressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase.
  • Post-translational
    modifications
    Phosphorylated during mitosis.
    Ubiquitinated, and this requires FZR1/CDH1.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein scraps homolog Drosophila antibody
    • Actin binding protein anillin antibody
    • Actin-binding protein anillin antibody
    • Anillin actin binding protein antibody
    • anln antibody
    • ANLN_HUMAN antibody
    • DKFZp779A055 antibody
    • Scra antibody
    • Scraps antibody
    • Scraps homolog antibody
    see all

Anti-Anillin antibody images

  • ab99352 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99352 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Anillin antibody (ab99352) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 124 kDa


    Exposure time : 30 seconds
  • Detection of Human Anillin by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). ab99352 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). Anillin was also immunoprecipitated by another rabbit anti-Anillin antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 3 seconds

References for Anti-Anillin antibody (ab99352)

ab99352 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99352.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"