Anti-Anillin antibody - C-terminal (ab154337)

Overview

  • Product name
    Anti-Anillin antibody - C-terminal
    See all Anillin primary antibodies
  • Description
    Rabbit polyclonal to Anillin - C-terminal
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 1062-1124 of Human Anillin (Uniprot ID: Q9NQW6).

  • Positive control
    • 293T and NIH 3T3 whole cell lysates; HeLa cells; Mouse brain, Human U87 xenograft and kidney tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab154337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 124 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
  • Tissue specificity
    Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
  • Sequence similarities
    Contains 1 PH domain.
  • Developmental stage
    Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase.
  • Post-translational
    modifications
    Phosphorylated during mitosis.
    Ubiquitinated, and this requires FZR1/CDH1.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein scraps homolog Drosophila antibody
    • Actin binding protein anillin antibody
    • Actin-binding protein anillin antibody
    • Anillin actin binding protein antibody
    • anln antibody
    • ANLN_HUMAN antibody
    • DKFZp779A055 antibody
    • FSGS8 antibody
    • Scra antibody
    • Scraps antibody
    • Scraps homolog antibody
    see all

Images

  • Anti-Anillin antibody - C-terminal (ab154337) at 1/500 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 124 kDa
  • Anti-Anillin antibody - C-terminal (ab154337) at 1/500 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 124 kDa
  • Confocal immunofluorescence analysis of methanol-fixed HeLa cells, labeling Anillin with ab154337 at 1/500 dilution (green). Alpha-tubulin filaments are labeled in red.
  • Immunohistochemical analysis of Paraffin-embedded Human U87 xenograft tissue, labeling Anillin with ab154337 at 1/500 dilution.
  • Immunohistochemical analysis of Paraffin-embedded Mouse brain tissue, labeling Anillin with ab154337 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human Kidney tissue, labeling Anillin with ab154337 at 1/100 dilution.

References

ab154337 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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