Overview

  • Product name
    Anti-ANKAR antibody
  • Description
    Rabbit polyclonal to ANKAR
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: QLPPAYYDTR IGQILINIDY MLKALWHGIY MPKEKRARFS ELWRAIMDID PDGKPQTNKD IFSEFSSAGL TDITKDPDFN EIYDEDVNED P, corresponding to amino acids 133-223 of Human ANKAR (Q7Z5J8).

  • Positive control
    • Human pancreas tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in centrosome. Recommended concentration of ab122319 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human pancreas with ab122319 at 1/125 dilution showing strong nuclear and cytoplasmic positivity in exocrine glandular cells.

References

ab122319 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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