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Rabbit polyclonal to ANKAR
antigen: QLPPAYYDTR IGQILINIDY MLKALWHGIY MPKEKRARFS ELWRAIMDID PDGKPQTNKD IFSEFSSAGL TDITKDPDFN EIYDEDVNED P, corresponding to amino acids 133-223 of Human ANKAR (Q7Z5J8).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Ubiquitously expressed with highest level in pancreas and lowest in skeletal muscle.
Contains 5 ANK repeats.
Contains 6 ARM repeats.
Information by UniProt
Ankyrin and armadillo repeat-containing protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-ANKAR antibody (ab122319)
Immunofluorescent staining of Human cell line A-431 shows positivity in centrosome. Recommended concentration of ab122319 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKAR antibody (ab122319)
Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human pancreas with ab122319 at 1/125 dilution showing strong nuclear and cytoplasmic positivity in exocrine glandular cells.
has not yet been referenced specifically in any publications.
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