Craniometaphyseal dysplasia Jackson type (dominant) antibody
Progressive ankylosis protein homolog antibody
Western blot - ANKH antibody (ab90104)
Anti-ANKH antibody (ab90104) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP-1 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 54 kDa
Immunocytochemistry/ Immunofluorescence - ANKH antibody (ab90104)This image is courtesy of an anonymous Abreview
ab90104 staining ANKH in Mouse pre-osteoblast MC3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin, permeabilized with TritonX and blocked with 3% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 in 0.5% serum + PBS) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
Du G et al. Abnormal Mechanical Loading Induces Cartilage Degeneration by Accelerating Meniscus Hypertrophy and Mineralization After ACL Injuries In Vivo. Am J Sports Med44:652-63 (2016).
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