Overview

  • Product name
  • Description
    Rabbit polyclonal to ANKIB1
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 925 and 975 of Human ANKIB11 (NP_061877.1).

  • Positive control
    • HeLa cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab99345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.
    • Sequence similarities
      Belongs to the RBR family.
      Contains 2 ANK repeats.
      Contains 1 IBR-type zinc finger.
      Contains 2 RING-type zinc fingers.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
    • Information by UniProt
    • Database links
    • Alternative names
      • AKIB1_HUMAN antibody
      • Ankib1 antibody
      • ankyrin repeat and IBR domain containing 1 antibody
      • Ankyrin repeat and IBR domain-containing protein 1 antibody
      • FLJ33123 antibody
      see all

    Images

    • 3µg ab99345 were used to immunoprecipitate ANKIB1 from 1 mg HeLa whole cell lysate.
      20% IP was loaded on gel and probed with a different anti-ANKIB1 antibody.
      Detection: chemiluminescence with an exposure time of 3 seconds.

    References

    ab99345 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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