Overview

  • Product name
  • Description
    Rabbit polyclonal to ANKIB1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 1039 and 1089 of Human ANKIB1 (NP_061877.1)

  • Positive control
    • HeLa or 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab99346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.
  • Sequence similarities
    Belongs to the RBR family.
    Contains 2 ANK repeats.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKIB1_HUMAN antibody
    • Ankib1 antibody
    • ankyrin repeat and IBR domain containing 1 antibody
    • Ankyrin repeat and IBR domain-containing protein 1 antibody
    • FLJ33123 antibody
    see all

Images

  • All lanes : Anti-ANKIB1 antibody (ab99346) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 122 kDa


    Exposure time : 30 seconds
  • Lane 1: 3µg ab99346 were used to precipitate ANKIB1 from 3mg HeLa whole cell lysate. 20% IP was loaded on gel and probed with ab99346 at 1µg/ml.
    Lane 2: same, except IP was carried with a control IgG.
    Detection: chemiluminescence with exposure times of 3 seconds.

References

ab99346 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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