ankyrin repeat and IBR domain containing 1 antibody
Ankyrin repeat and IBR domain-containing protein 1 antibody
Western blot - ANKIB1 antibody (ab99346)
All lanes : Anti-ANKIB1 antibody (ab99346) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 122 kDa
Exposure time : 30 seconds
Immunoprecipitation - ANKIB1 antibody (ab99346)
Lane 1: 3µg ab99346 were used to precipitate ANKIB1 from 3mg HeLa whole cell lysate. 20% IP was loaded on gel and probed with ab99346 at 1µg/ml.
Lane 2: same, except IP was carried with a control IgG.
Detection: chemiluminescence with exposure times of 3 seconds.