Overview

  • Product nameAnti-ANKRA2 antibody
    See all ANKRA2 primary antibodies
  • Description
    Rabbit polyclonal to ANKRA2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (LLYAVHGNHV KCVKMLLESG ADPTIETDSG YNSMDLAVAL GYRSVQQVIE) of Human ANKRA2 (NP_075526).

  • Positive control
    • HeLa cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab81201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • FunctionMay facilitate endocytosis by linking megalin to components of the cytoskeleton or endocytic machinery.
  • Sequence similaritiesContains 3 ANK repeats.
  • Cellular localizationCytoplasm > cytoskeleton. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANKRA 2 antibody
    • ANKRA antibody
    • ANKRA2 antibody
    • Ankyrin repeat family A (RFXANK like) 2 antibody
    • Ankyrin repeat family A 2 antibody
    • Ankyrin repeat family A protein 2 antibody
    • Ankyrin repeat-containing protein, family A, member 2 antibody
    • ANRA2_HUMAN antibody
    • C11 - RNP3 antibody
    • C11 antibody
    • hRPC11 antibody
    • My010 antibody
    • OTTHUMP00000128195 antibody
    • RFXANK like 2 antibody
    • RFXANK-like protein 2 antibody
    • RPC10 antibody
    • RPC11 antibody
    • RPC12.5 antibody
    see all

Anti-ANKRA2 antibody images

  • Anti-ANKRA2 antibody (ab81201) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ANKRA2 antibody (ab81201)

ab81201 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"