Anti-ANKRD11 antibody [2022C8a] (ab50852)

Overview

  • Product name
    Anti-ANKRD11 antibody [2022C8a]
    See all ANKRD11 primary antibodies
  • Description
    Mouse monoclonal [2022C8a] to ANKRD11
  • Specificity
    This antibody reacts with ANKRD11
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, African green monkey
  • Immunogen

    ANKRD11 recombinant fragment (Human), containing 50-200 aa

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% PBS, BSA, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Protein G purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum.
  • Clonality
    Monoclonal
  • Clone number
    2022C8a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 296 kDa.

Target

  • Relevance
    Ankyrin is a membrane protein that mediates the attachment of the erythrocyte membrane skeleton to the plasma membrane and interacts with CD44 and inositol triphosphate. It contains three functional domains: a conserved N-terminal ankyrin repeat domain (ARD(consisting of 22–24 tandem repeats of 33 amino acids), a spectrin binding domain and a variably sized C-terminal regulatory domain. The ankyrin repeat is a 33-residue motif in proteins consisting of two alpha helices separated by loops. It has been studied using multiple sequence alignment to determine which conserved amino acid residues are critical for folding and stability. Ankyrin-repeat proteins have been associated with a number of human diseases; most notably, the cell cycle inhibitor p16 is associated with cancer and the Notch protein is a key component of cell signaling pathways whose intracellular repeat domain is disrupted in mutations that give rise to the neurological disorder known as CADASIL.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • ANCO 1 antibody
    • ANCO1 antibody
    • Ankyrin repeat containing cofactor 1 antibody
    • Ankyrin repeat domain 11 antibody
    • Ankyrin repeat domain containing protein 11 antibody
    • LZ16 antibody
    • T13 antibody
    see all

Images

  • Anti-ANKRD11 antibody [2022C8a] (ab50852) at 1/50 dilution + A2058 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 2500

    Predicted band size : 296 kDa
    Observed band size : >250 kDa (why is the actual band size different from the predicted?)

References

ab50852 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount
30 µg
Specification
U2OS and 293T cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Nov 13 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 13 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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