Anti-ANKRD11 antibody [2022C8a] (ab50852)
Key features and details
- Mouse monoclonal [2022C8a] to ANKRD11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ANKRD11 antibody [2022C8a] -
Description
Mouse monoclonal [2022C8a] to ANKRD11 -
Host species
Mouse -
Specificity
This antibody reacts with ANKRD11.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ANKRD11 aa 1463-1585.
Sequence:EKHREKWRDE KERHRDRHAD GLLRHHRDEL LRHHRDEQKP ATRDKDSPPR VLKDKSRDEG PRLGDAKLKE KFKDGAEKEK GDPVKMSNGN DKVAPSKDPG KKDARPREKL LGDGDLMMTS FER
Database link: Q6UB99 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: BSA, 1% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Protein G purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
2022C8a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/50. Predicted molecular weight: 296 kDa.
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Notes |
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WB
1/50. Predicted molecular weight: 296 kDa. |
Target
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Relevance
Ankyrin is a membrane protein that mediates the attachment of the erythrocyte membrane skeleton to the plasma membrane and interacts with CD44 and inositol triphosphate. It contains three functional domains: a conserved N-terminal ankyrin repeat domain (ARD(consisting of 22–24 tandem repeats of 33 amino acids), a spectrin binding domain and a variably sized C-terminal regulatory domain. The ankyrin repeat is a 33-residue motif in proteins consisting of two alpha helices separated by loops. It has been studied using multiple sequence alignment to determine which conserved amino acid residues are critical for folding and stability. Ankyrin-repeat proteins have been associated with a number of human diseases; most notably, the cell cycle inhibitor p16 is associated with cancer and the Notch protein is a key component of cell signaling pathways whose intracellular repeat domain is disrupted in mutations that give rise to the neurological disorder known as CADASIL. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 29123 Human
- SwissProt: Q6UB99 Human
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Alternative names
- ANCO 1 antibody
- ANCO1 antibody
- Ankyrin repeat containing cofactor 1 antibody
see all
Images
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Anti-ANKRD11 antibody [2022C8a] (ab50852) at 1/50 dilution + A2058 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody
Predicted band size: 296 kDa
Observed band size: >250 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (2)
ab50852 has been referenced in 2 publications.
- Cucco F et al. Pathogenic variants in EP300 and ANKRD11 in patients with phenotypes overlapping Cornelia de Lange syndrome. Am J Med Genet A 182:1690-1696 (2020). PubMed: 32476269
- Garee JP et al. Regulation of HER2 oncogene transcription by a multifunctional coactivator/corepressor complex. Mol Endocrinol 28:846-59 (2014). PubMed: 24678732