Overview

  • Product name
    Anti-ANKRD12 antibody
  • Description
    Rabbit polyclonal to ANKRD12
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: NEERDSDTDS DPGHTSENWG ERLISSYRTY SEKEGPEKKK TKKEAGNKKS, corresponding to N terminal amino acids 71-120 of Human ANKRD12

  • Positive control
    • HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 236 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    Ankyrin repeats containing cofactors may recruit histone deacetylases to the p160 coactivators/nuclear receptor complex to inhibit ligand-dependent transactivation.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • ANCO 2 antibody
    • Ankyrin repeat domain-containing protein 12 antibody
    • Ankyrin repeat-containing cofactor 2 antibody
    • FLJ20053 antibody
    • GAC 1 antibody
    • GAC 1 protein antibody
    • HGNC:29135 antibody
    • KIAA0874 antibody
    see all

Images

  • Anti-ANKRD12 antibody (ab66309) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 236 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References

ab66309 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66309.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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