Overview

  • Product name
  • Description
    Rabbit polyclonal to ANKRD17
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 775 and 825 of Human ANKRD17 (NP_115593.3).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab85726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 274 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Could play pivotal roles in cell cycle and DNA regulation (PubMed:19150984). Involved in innate immune defense against viruse by positively regulating the viral dsRNA receptors DDX58 and IFIH1 signaling pathways (PubMed:22328336). Involves in NOD2- and NOD1-mediated responses to bacteria suggesting a role in innate antibacterial immune pathways too (PubMed:23711367). Target of enterovirus 71 which is the major etiological agent of HFMD (hand, foot and mouth disease) (PubMed:17276651). Could play a central role for the formation and/or maintenance of the blood vessels of the circulation system.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Contains 25 ANK repeats.
    Contains 1 KH domain.
  • Post-translational
    modifications
    Phosphorylated by CDK2.
  • Cellular localization
    Cytoplasm. Nucleus. Detected around the nucleolus. Localized on chromatin in a cell cycle-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANKRD 17 antibody
    • ANKRD17 antibody
    • Ankyrin repeat domain protein 17 antibody
    • Ankyrin repeat domain-containing protein 17 antibody
    • ANR17_HUMAN antibody
    • FLJ22206 antibody
    • Gene trap ankyrin repeat antibody
    • Gene trap ankyrin repeat protein antibody
    • GTAR antibody
    • KIAA0697 antibody
    • MASK2 antibody
    • NY BR 16 antibody
    • NY-BR-16 antibody
    • Serologically defined breast cancer antigen NY-BR-16 antibody
    see all

Images

  • All lanes : Anti-ANKRD17 antibody (ab85726) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 274 kDa
    Observed band size : 274 kDa
    Additional bands at : 268 kDa,71 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human ANKRD17 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85726 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab85726 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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