Overview

  • Product name
  • Description
    Rabbit polyclonal to ANKRD23
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: KRLRHRVPPR KPEPLVKPQS QAQVEPVGLE MFLKAAAENQ EYLIDKYLTD GGDPNAHDKL HRTALHWAC, corresponding to amino acids 84-152 of Human ANKRD23 (Q86SG2).

  • Positive control
    • A-431 cells, human smooth muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • Function
    May be involved in the energy metabolism. Could be a molecular link between myofibrillar stretch-induced signaling pathways and muscle gene expression.
  • Tissue specificity
    Mainly expressed in heart, skeletal muscle and brown adipose tissues.
  • Sequence similarities
    Contains 4 ANK repeats.
  • Cellular localization
    Nucleus. Sarcomeric I-band and some intercalated disks.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANKRD23 antibody
    • Ankyrin repeat domain 23 antibody
    • Ankyrin repeat domain-containing protein 23 antibody
    • ANR23_HUMAN antibody
    • DARP antibody
    • Diabetes-related ankyrin repeat protein antibody
    • FLJ32449 antibody
    • MARP3 antibody
    • MGC129593 antibody
    • Muscle ankyrin repeat protein 3 antibody
    • OTTHUMP00000161094 antibody
    • OTTHUMP00000207608 antibody
    • OTTHUMP00000207609 antibody
    see all

Images

  • Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human smooth muscle with ab122320 at 1/250 dilution showing strong cytoplasmic positivity in smooth muscle cells
  • All lanes : Anti-ANKRD23 antibody (ab122320) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
  • Immunofluorescent staining of human cell line A-431 (Fixation/Permeabilization: PFA/Triton X-100) with ab122320 at 4ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.

References

ab122320 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (whole mouse heart)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
whole mouse heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 16 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - 0.15% Triton-X-100
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted May 12 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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