Anti-KANK2 antibody (ab99351)
Key features and details
- Rabbit polyclonal to KANK2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KANK2 antibody
See all KANK2 primary antibodies -
Description
Rabbit polyclonal to KANK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human KANK2 aa 550-650.
Database link: Q63ZY3 -
Positive control
- HeLa whole cell lysate (ab150035)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: PBS, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
|
IP |
Use a concentration of 2 - 5 µg/ml.
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|
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 91 kDa. |
IP
Use a concentration of 2 - 5 µg/ml. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
KANK2 contains 5 ANK repeats. It is strongly expressed in cervix, colon, heart, kidney and lung. -
Cellular localization
Cytoplasm. Mitochondrion. -
Database links
- Entrez Gene: 25959 Human
- Omim: 614610 Human
- SwissProt: Q63ZY3 Human
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Alternative names
- ANKRD25 antibody
- Ankyrin repeat domain 25 antibody
- Ankyrin repeat domain containing protein 25 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling KANK2 with ab99351 at 1/1000 (1µg/ml). Detection: DAB.
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All lanes : Anti-KANK2 antibody (ab99351) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 3 minutes -
Detection of Human KANK2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). ab99351 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). KANK2 was also immunoprecipitated by another rabbit anti-KANK2 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 30 seconds.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab99351 has not yet been referenced specifically in any publications.