Overview

  • Product name
  • Description
    Rabbit polyclonal to ANKRD25
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 550 - 600 of Human ANKRD25 (NP_056308.2)

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, Tris buffered saline, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab99351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
IP Use a concentration of 2 - 5 µg/ml.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    ANKRD25 contains 5 ANK repeats. It is strongly expressed in cervix, colon, heart, kidney and lung.
  • Cellular localization
    Cytoplasm. Mitochondrion.
  • Database links
  • Alternative names
    • ANKRD25 antibody
    • Ankyrin repeat domain 25 antibody
    • Ankyrin repeat domain containing protein 25 antibody
    • DKFZp434N161 antibody
    • FLJ20004 antibody
    • KANK 2 antibody
    • KANK2 antibody
    • KIAA1518 antibody
    • Kidney ankyrin repeat containing protein 2 antibody
    • KN motif and ankyrin repeat domain containing protein 2 antibody
    • KN motif and ankyrin repeat domains 2 antibody
    • Matrix remodeling associated protein 3 antibody
    • Matrix remodelling associated 3 antibody
    • MGC119707 antibody
    • MXRA3 antibody
    • SIP antibody
    • SRC 1 interacting protein antibody
    • SRC interacting protein antibody
    • SRC1 interacting protein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD25 with ab99351 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ANKRD25 antibody (ab99351) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 91 kDa


    Exposure time : 3 minutes
  • Detection of Human ANKRD25 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). ab99351 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). ANKRD25 was also immunoprecipitated by another rabbit anti-ANKRD25 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 30 seconds.

References

ab99351 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (primary myometrial cells)
Gel Running Conditions
Reduced Denaturing (7% Bis-Tris)
Loading amount
20 µg
Specification
primary myometrial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up