Overview

  • Product name
  • Description
    Rabbit polyclonal to Ankrd26
  • Specificity
    This antibody is specific for mouse Ankrd26.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide:

    MKKIFGFRSKGPSPL

    , corresponding to amino acids 1-15 of Mouse Ankrd26

  • Positive control
    • MES cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/80000.
WB 1/500 - 1/3000. Predicted molecular weight: 196 kDa.

Target

Images

  • Lane 1 :
    Lanes 2 - 10 : Anti-Ankrd26 antibody (ab47984) at 1/1000 dilution

    Lane 1 : BLANK
    Lane 2 : MES cell lysate - 80µg
    Lane 3 : MES cell lysate - 40µg
    Lane 4 : 293T-ANKRD26 transfected cell lysate - 20µg
    Lane 5 : control 293T cell lysate - 20µg
    Lane 6 : BSA-ANKRD26 conjugate - 20ng
    Lane 7 : BSA - 500ng
    Lane 8 : BSA - 100ng
    Lane 9 : BSA - 20ng
    Lane 10 : Protein standards

    Secondary
    Lanes 2 - 10 : ALP conjugated Gt-a-Rabbit IgG at 1/3000 dilution


    Predicted band size : 196 kDa
    Observed band size : 81 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Cardamone MD  et al. GPS2/KDM4A pioneering activity regulates promoter-specific recruitment of PPAR?. Cell Rep 8:163-76 (2014). Mouse . Read more (PubMed: 24953653) »

See 1 Publication for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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