Overview

  • Product name
  • Description
    Rabbit polyclonal to Ankrd26
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1659-1709 of Human Ankrd26 (NP_055730.1)

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 10µg/mg of lysate.
    WB: 1/1000 - 1/5000 (or 0.4 - 1µg/ml). Predicted molecular weight: 196 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similarities
      Contains 5 ANK repeats.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ankrd26 antibody
      • Ankyrin repeat domain 26 antibody
      • Ankyrin repeat domain-containing protein 26 antibody
      • Ankyrin repeat domaincontaining protein 26 antibody
      • ANR26_HUMAN antibody
      • bA145E8.1 antibody
      • KIAA1074 antibody
      see all

    Images

    • HeLa whole cell lysate (1 mg) was immunoprecipitated using 10 µg ab86780 (lane 1), or a control IgG antibody (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab86780 at 1 µg/ml. Bands were detected using chemiluminescence with an exposure time of 30 seconds.
    • All lanes : Anti-Ankrd26 antibody (ab86780) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 196 kDa


      Exposure time : 3 minutes

    References

    ab86780 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab86780.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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