Overview

  • Product nameAnti-ANKRD28 antibody
    See all ANKRD28 primary antibodies
  • Description
    Rabbit polyclonal to ANKRD28
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide from a region between residue 975 and 1025 of human ANKRD28, NP_056014.2.

  • Positive control
    • Whole cell lysate from Hela and 293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 117, 240, 360 kDa (predicted molecular weight: 117 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • RelevanceANKRD28 selectively inhibits the phosphatase activity of PPP1C and targets PPP1C to modulate HNRPK phosphorylation. It contains twenty seven ANK repeats. There are two named isoforms.
  • Cellular localizationNucleus, nucleoplasm. Note: Seems to be excluded from nucleoli.
  • Database links
  • Alternative names
    • AI465466 antibody
    • AI604979 antibody
    • ANKRD 28 antibody
    • Ankrd28 antibody
    • Ankyrin repeat domain 28 antibody
    • E430019N21Rik antibody
    • KIAA0379 antibody
    • mKIAA0379 antibody
    • Phosphatase interactor targeting K protein antibody
    • Phosphatase interactor targeting protein hnRNP K antibody
    • PITK antibody
    • PPP1R65 antibody
    • RGD1559931 antibody
    see all

Anti-ANKRD28 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD28 with ab72037 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-ANKRD28 antibody (ab72037) at 0.02 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 117 kDa
    Observed band size : 117 kDa
    Additional bands at : 240 kDa,360 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of ANKRD28.
    Lane 1: ab72037 at 3µg/mg whole cell lysate for IP followed by ab72037 at 1µg/ml for subsequent WB.
    Lane 2: Control IgG.

    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:chemiluminescence with an exposure time of 10 seconds.

References for Anti-ANKRD28 antibody (ab72037)

ab72037 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"