Overview

  • Product name
  • Description
    Rabbit polyclonal to ANKRD28
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide from a region between residue 975 and 1025 of human ANKRD28, NP_056014.2.

  • Positive control
    • Whole cell lysate from Hela and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 117, 240, 360 kDa (predicted molecular weight: 117 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance
    ANKRD28 selectively inhibits the phosphatase activity of PPP1C and targets PPP1C to modulate HNRPK phosphorylation. It contains twenty seven ANK repeats. There are two named isoforms.
  • Cellular localization
    Nucleus, nucleoplasm. Note: Seems to be excluded from nucleoli.
  • Database links
  • Alternative names
    • AI465466 antibody
    • AI604979 antibody
    • ANKRD 28 antibody
    • Ankrd28 antibody
    • Ankyrin repeat domain 28 antibody
    • E430019N21Rik antibody
    • KIAA0379 antibody
    • mKIAA0379 antibody
    • Phosphatase interactor targeting K protein antibody
    • Phosphatase interactor targeting protein hnRNP K antibody
    • PITK antibody
    • PPP1R65 antibody
    • RGD1559931 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD28 with ab72037 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-ANKRD28 antibody (ab72037) at 0.02 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 117 kDa
    Observed band size : 117 kDa
    Additional bands at : 240 kDa,360 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of ANKRD28.
    Lane 1: ab72037 at 3µg/mg whole cell lysate for IP followed by ab72037 at 1µg/ml for subsequent WB.
    Lane 2: Control IgG.

    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:chemiluminescence with an exposure time of 10 seconds.

References

ab72037 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab72037.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up