Overview

  • Product nameAnti-ANKRD34A antibody
    See all ANKRD34A primary antibodies
  • Description
    Rabbit polyclonal to ANKRD34A
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: KPLKRLNSEP WGLVAPPQPV PPTEGRPGIE RLTAEFNGLT LTGRPRLSRR HSTEGPEDPP PWAEKVTSGG PLSRRNTAPE AQESGP, corresponding to amino acids 240-325 of Human ANKRD34A.

  • Positive control
    • Human Duodenum tissue; RT-4 and U-251 MG cell lysates; Human Liver and Tonsil lysates; Human U-2 OS cell line

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-ANKRD34A antibody images

  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • ab122073, at 1/60 dilution, staining ANKRD34A in paraffin-embedded Human Duodenum tissue by Immmunohistochemistry.
  • ab122073, at 4µ/ml, staining ANKRD34A in U-2 OS cells by Immunofluorescence. Antibody staining is shown in green.

  • All lanes : Anti-ANKRD34A antibody (ab122073) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

References for Anti-ANKRD34A antibody (ab122073)

ab122073 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"