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- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
- PurityImmunogen affinity purified
- Research areas
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- Sequence similaritiesContains 6 ANK repeats.
Information by UniProt
- ANKRD35 antibody
- Ankyrin repeat domain 35 antibody
- Ankyrin repeat domain-containing protein 35 antibody
- ANR35_HUMAN antibody
Anti-ANKRD35 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-ANKRD35 antibody (ab122279)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab122279 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-ANKRD35 antibody (ab122279)
All lanes : Anti-ANKRD35 antibody (ab122279) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U251-MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD35 antibody (ab122279)
ab122279, at 1/250 dilution, staining ANKRD35 in paraffin-embedded Human nasopharynx tissue by
References for Anti-ANKRD35 antibody (ab122279)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"