Overview

  • Product name
  • Description
    Rabbit polyclonal to ANKRD35
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: QLREELAAVW REKDAARGAL SRPVMEGALG TPRAEAAAAA WEKMEARLER VLARLEWAKA GLQVKPEVPS QESREGALKA APGSIKQDEE KEKRVPGA, corresponding to amino acids 494-591 of Human ANKRD35.

  • Positive control
    • RT-4 and U251-MG cell lysates; Human tonsil lysate; Human nasopharynx tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab122279 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-ANKRD35 antibody (ab122279) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U251-MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • ab122279, at 1/250 dilution, staining ANKRD35 in paraffin-embedded Human nasopharynx tissue by Immunohistochemistry.

References

ab122279 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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