Overview

  • Product name
    Anti-ANKRD50 antibody
  • Description
    Rabbit polyclonal to ANKRD50
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1379 and 1429 of Human ANKRD50 (NP_065070.1).

  • Positive control
    • HeLa, 293T and NIH 3T3 whole cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab108219 was affinity purified using an epitope specific to ANKRD50 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 156 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

Images

  • All lanes : Anti-ANKRD50 antibody (ab108219) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 156 kDa


    Exposure time : 30 seconds
  • ab108219 at 1 µg/ml detecting ANKRD50 in HeLa whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes an upstream epitope of ANKRD50
    Lane 2: ab108219 at 6 µg/mg of lysate
    Lane 3: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 10 seconds

References

This product has been referenced in:
  • McGough IJ  et al. Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling. J Cell Sci 127:4940-53 (2014). Read more (PubMed: 25278552) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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