Overview

  • Product name
  • Description
    Rabbit polyclonal to ANKRD56
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LDEGLLKRSR RPPRSRKPSK AGTAPSPRVD AGLSLKLAEV KAVVAERGWR HSLWVPSGEG SAALAPHRTS EHKSSLVPLD AREHEWIVKL ASGS, corresponding to amino acids 515-608 of Human ANKRD56 (UniProt ID: A6NEL2).

  • Positive control
    • IHC-P: Human cerebellum tissue WB: RT-4 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities
    Contains 2 ANK repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANKRD56 antibody
    • Ankyrin repeat domain 56 antibody
    • Ankyrin repeat domain containing protein SOWAHB antibody
    • Ankyrin repeat domain-containing protein 56 antibody
    • ANR56_HUMAN antibody
    • Protein sosondowah homolog B antibody
    • Sosondowah ankyrin repeat domain family member B antibody
    • SOWAHB antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab122369 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122369, at a 1/50 dilution, staining ANKRD56 in paraffin embedded Human cerebellum tissue by immunohistochemistry.
  • All lanes : Anti-ANKRD56 antibody (ab122369) at 1/250 dilution

    Lane 1 : Marker
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human plasma lysate
    Lane 5 : Human liver lysate
    Lane 6 : Human tonsil lysate

References

ab122369 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab122369.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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