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Rabbit polyclonal to ANKRD56
antigen sequence: LDEGLLKRSR RPPRSRKPSK AGTAPSPRVD AGLSLKLAEV KAVVAERGWR HSLWVPSGEG SAALAPHRTS EHKSSLVPLD AREHEWIVKL ASGS, corresponding to amino acids 515-608 of Human ANKRD56 (UniProt ID: A6NEL2).
- IHC-P: Human cerebellum tissue
WB: RT-4 cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Contains 2 ANK repeats.
Information by UniProt
- ANKRD56 antibody
- Ankyrin repeat domain 56 antibody
- Ankyrin repeat domain containing protein SOWAHB antibody
Immunocytochemistry/ Immunofluorescence - Anti-ANKRD56 antibody (ab122369)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab122369 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD56 antibody (ab122369)
ab122369, at a 1/50 dilution, staining ANKRD56 in paraffin embedded Human cerebellum tissue by immunohistochemistry.
Western blot - Anti-ANKRD56 antibody (ab122369)
All lanes : Anti-ANKRD56 antibody (ab122369) at 1/250 dilution
Lane 1 : Marker
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human plasma lysate
Lane 5 : Human liver lysate
Lane 6 : Human tonsil lysate
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"