Overview

  • Product name
  • Description
    Rabbit polyclonal to ANKS1A
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 875 and 925 of Human ANKS1A (NP_056060.2).

  • Positive control
    • Whole cell lysate from HeLa cells and 293T.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 123 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    May play a negative role in growth factor receptor signaling pathways.
  • Tissue specificity
    Widely expressed (at protein level).
  • Sequence similarities
    Contains 6 ANK repeats.
    Contains 1 PID domain.
    Contains 2 SAM (sterile alpha motif) domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues in response to EGF and PDGF.
  • Cellular localization
    Cytoplasm. Cytoplasmic before and after growth factor treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANKS 1 antibody
    • ANKS 1A antibody
    • ANKS1 antibody
    • ANKS1A antibody
    • Ankyrin repeat and SAM domain containing 1 antibody
    • Ankyrin repeat and SAM domain containing protein 1A antibody
    • Ankyrin repeat and SAM domain-containing protein 1A antibody
    • Ankyrin repeat and sterile alpha motif domain containing 1A antibody
    • ANS1A_HUMAN antibody
    • KIAA0229 antibody
    • MGC42354 antibody
    • Odin antibody
    see all

Images

  • All lanes : Anti-ANKS1A antibody (ab112001) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 123 kDa


    Exposure time : 3 minutes
  • Detection of ANKS1A in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab112001 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab112001 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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