Overview

  • Product nameAnti-ANKS1A antibody
    See all ANKS1A primary antibodies
  • Description
    Mouse polyclonal to ANKS1A
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-460 of Human ANKS1A

  • Positive control
    • ANKS1A transfected 293T cell lysate. HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 123 kDa).

    Note: This product requires purification with Protein A prior to ICC/IF.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a negative role in growth factor receptor signaling pathways.
    • Tissue specificityWidely expressed (at protein level).
    • Sequence similaritiesContains 6 ANK repeats.
      Contains 1 PID domain.
      Contains 2 SAM (sterile alpha motif) domains.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues in response to EGF and PDGF.
    • Cellular localizationCytoplasm. Cytoplasmic before and after growth factor treatment.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANKS 1 antibody
      • ANKS 1A antibody
      • ANKS1 antibody
      • ANKS1A antibody
      • Ankyrin repeat and SAM domain containing 1 antibody
      • Ankyrin repeat and SAM domain containing protein 1A antibody
      • Ankyrin repeat and SAM domain-containing protein 1A antibody
      • Ankyrin repeat and sterile alpha motif domain containing 1A antibody
      • ANS1A_HUMAN antibody
      • KIAA0229 antibody
      • MGC42354 antibody
      • Odin antibody
      see all

    Anti-ANKS1A antibody images

    • All lanes : Anti-ANKS1A antibody (ab67533) at 1/500 dilution

      Lane 1 : ANKS1A transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 123 kDa
      Observed band size : 78 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of ANKS1A in HeLa cells using purified ab67533 at 10 ug/ml.

    References for Anti-ANKS1A antibody (ab67533)

    ab67533 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab67533.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"