Overview

  • Product nameAnti-Annexin A1 antibody [5E4/1]See all Annexin A1 primary antibodies ...
  • Description
    Mouse monoclonal [5E4/1] to Annexin A1
  • Tested applicationsELISA, WB, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Horse, Dog, Human
  • Immunogen

    Mixture of native membrane proteins from human bone tissues. This includes native whole annexin proteins.

  • Positive control
    • Endothelial cells

Properties

Applications

Our Abpromise guarantee covers the use of ab2487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 38.5 kDa.
ICC/IF Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 22384219

Target

  • FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
  • Cellular localizationNucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin 1 antibody
    • Annexin A1 antibody
    • Annexin I (lipocortin I) antibody
    • Annexin I antibody
    • Annexin-1 antibody
    • AnnexinA1 antibody
    • AnnexinI antibody
    • ANX 1 antibody
    • ANX A1 antibody
    • ANX1 antibody
    • ANXA 1 antibody
    • ANXA1 antibody
    • ANXA1 protein antibody
    • ANXA1_HUMAN antibody
    • Calpactin 2 antibody
    • Calpactin II antibody
    • Calpactin-2 antibody
    • CalpactinII antibody
    • Chromobindin 9 antibody
    • Chromobindin-9 antibody
    • Chromobindin9 antibody
    • HGNC:533 antibody
    • Lipocortin 1 antibody
    • Lipocortin I antibody
    • Lipocortin1 antibody
    • LipocortinI antibody
    • LPC 1 antibody
    • LPC1 antibody
    • p35 antibody
    • Phospholipase A2 inhibitory protein antibody
    see all

Anti-Annexin A1 antibody [5E4/1] images

  • ab2487, staining Annexin A1 (green) in and sub-acutely injured equine superficial digital flexor tendons by Immunohistochemistry (Frozen sections).

    After warming cryosections at room temperature for 30 minutes, sections were rehydrated in 1% PBS-Tween 20 (PBS-T) and blocked in PBS containing 5% normal goat serum for 1 hour at room temperature. Sections were incubated with the primary antibody (1/100 in PBS + 5% normal goat serum) for 2 hours. An AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/300).
  • Immunofluorescence analysis of MDCK cells, staining Annexin A1 with ab2487.

References for Anti-Annexin A1 antibody [5E4/1] (ab2487)

This product has been referenced in:
  • Sena A  et al. Dysregulation of anti-inflammatory annexin A1 expression in progressive crohns disease. PLoS One 8:e76969 (2013). IHC-Fr ; Human . Read more (PubMed: 24130820) »
  • Xiao H  et al. Proteomic analysis of human saliva from lung cancer patients using two-dimensional difference gel electrophoresis and mass spectrometry. Mol Cell Proteomics 11:M111.012112 (2012). Read more (PubMed: 22096114) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Oral squamous cell carcinoma)
Specification Oral squamous cell carcinoma
Fixative Paraformaldehyde
Antigen retrieval step Enzymatic
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2%
Username

Mr. Cancer center

Verified customer

Submitted Jul 27 2006

Thank you for your enquiry. To our knowledge, these antibodies have yet to be tested in IHC-P. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how you g...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"