Overview

  • Product name
  • Description
    Rabbit polyclonal to Annexin A1
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 24-346 of Human Annexin A1 (Uniprot P04083)

  • Positive control
    • A431, H1299, HeLa, MOLT4, NIH-3T3 and JC whole cell lysates; HeLa cells; N87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.

Target

  • Function
    Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
  • Cellular localization
    Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin 1 antibody
    • Annexin A1 antibody
    • Annexin I (lipocortin I) antibody
    • Annexin I antibody
    • Annexin-1 antibody
    • AnnexinA1 antibody
    • AnnexinI antibody
    • ANX 1 antibody
    • ANX A1 antibody
    • ANX1 antibody
    • ANXA 1 antibody
    • ANXA1 antibody
    • ANXA1 protein antibody
    • ANXA1_HUMAN antibody
    • Calpactin 2 antibody
    • Calpactin II antibody
    • Calpactin-2 antibody
    • CalpactinII antibody
    • Chromobindin 9 antibody
    • Chromobindin-9 antibody
    • Chromobindin9 antibody
    • HGNC:533 antibody
    • Lipocortin 1 antibody
    • Lipocortin I antibody
    • Lipocortin1 antibody
    • LipocortinI antibody
    • LPC 1 antibody
    • LPC1 antibody
    • p35 antibody
    • Phospholipase A2 inhibitory protein antibody
    see all

Images

  • All lanes : Anti-Annexin A1 antibody (ab137745) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : H1299 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 39 kDa
  • All lanes : Anti-Annexin A1 antibody (ab137745) at 1/10000 dilution

    Lane 1 : NIH-3T3 cell lysate
    Lane 2 : JC cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 39 kDa
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Annexin A1 with ab137745 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded N87 Xenograft, labelling Annexin A1 using ab137745 at 1/500 dilution.

References

This product has been referenced in:
  • Fang Y  et al. Knockdown of ANXA1 suppresses the biological behavior of human NSCLC cells in vitro. Mol Med Rep 13:3858-66 (2016). WB ; Human . Read more (PubMed: 27035116) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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