Overview

  • Product name
  • Description
    Rabbit polyclonal to Annexin A1
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Cow, Chimpanzee, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human Annexin A1 aa 1-346.
    Sequence:

    MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAA LHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKAL TGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIR DINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLAD SDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNK VLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIM VSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN


    Database link: P04083

  • Positive control
    • HeLa cells and cell lysates. NIH/3T3 cell lysates. Human endometrial cancer tissue, Human thyroid cancer tissue and Rat brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab196830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 39 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function
    Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
  • Cellular localization
    Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin 1 antibody
    • Annexin A1 antibody
    • Annexin I (lipocortin I) antibody
    • Annexin I antibody
    • Annexin-1 antibody
    • AnnexinA1 antibody
    • AnnexinI antibody
    • ANX 1 antibody
    • ANX A1 antibody
    • ANX1 antibody
    • ANXA 1 antibody
    • ANXA1 antibody
    • ANXA1 protein antibody
    • ANXA1_HUMAN antibody
    • Calpactin 2 antibody
    • Calpactin II antibody
    • Calpactin-2 antibody
    • CalpactinII antibody
    • Chromobindin 9 antibody
    • Chromobindin-9 antibody
    • Chromobindin9 antibody
    • HGNC:533 antibody
    • Lipocortin 1 antibody
    • Lipocortin I antibody
    • Lipocortin1 antibody
    • LipocortinI antibody
    • LPC 1 antibody
    • LPC1 antibody
    • p35 antibody
    • Phospholipase A2 inhibitory protein antibody
    see all

Images

  • All lanes : Anti-Annexin A1 antibody (ab196830) at 1/500 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : NIH/3T3 cell lysates


    Predicted band size : 39 kDa
  • Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Annexin A1 with ab196830 at 1/200 dilution (400x lens).

  • Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Annexin A1 with ab196830 at 1/200 dilution (200x lens).

  • Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Annexin A1 with ab196830 at 1/200 dilution (200x lens).

  • Immunofluorescent analysis of HeLa cells labeling Annexin A1 with ab196830 at 1/50 dilution. Blue: DAPI for nuclear staining.

References

ab196830 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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