Annexin A1 antibody (ab33061)

Product Name 

Annexin A1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Annexin A1

Immunogen 

Synthetic peptide: MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA, corresponding to a region within N terminal amino acids 1-50 of Human Annexin A1.
BLAST 'MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA ' with or

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/62500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 39 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: HepG2 cell lysate.
IHC-P: Human intestine.

Cellular localization 

Nucleus. Cytoplasm. Cell projection, cilium. Basolateral cell membrane. Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages.

Research areas 

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Other
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Annexins

Relevance 

This gene encodes a protein that belongs to a family of Ca(2+) dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti inflammatory activity.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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Annexin A1 antibody images:

References for Annexin A1 antibody (ab33061)

  This product has been used in: (two most recent references)

Carmichael ST et al. Genomic profiles of damage and protection in human intracerebral hemorrhage. J Cereb Blood Flow Metab : (2008). PubMed: 18628781

Cao Z et al. Use of fluorescence-activated vesicle sorting for isolation of Naked2-associated, basolaterally targeted exocytic vesicles for proteomics analysis. Mol Cell Proteomics 7:1651-67 (2008). PubMed: 18504258
If you publish research using ab33061 please let us know so that we can cite the reference on this datasheet.

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