Overview

  • Product name
  • Description
    Rabbit polyclonal to Annexin A1
  • Tested applications
    Suitable for: ICC, WB, IHC-P, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cat, Chimpanzee
  • Immunogen

    Synthetic peptide: MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA, corresponding to a region within N terminal amino acids 1-50 of Human Annexin A1.

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: Human intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab33061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration. PubMed: 20618702Fixed with acetone.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.
Flow Cyt Use at an assay dependent concentration. PubMed: 22378018ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
  • Cellular localization
    Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin 1 antibody
    • Annexin A1 antibody
    • Annexin I (lipocortin I) antibody
    • Annexin I antibody
    • Annexin-1 antibody
    • AnnexinA1 antibody
    • AnnexinI antibody
    • ANX 1 antibody
    • ANX A1 antibody
    • ANX1 antibody
    • ANXA 1 antibody
    • ANXA1 antibody
    • ANXA1 protein antibody
    • ANXA1_HUMAN antibody
    • Calpactin 2 antibody
    • Calpactin II antibody
    • Calpactin-2 antibody
    • CalpactinII antibody
    • Chromobindin 9 antibody
    • Chromobindin-9 antibody
    • Chromobindin9 antibody
    • HGNC:533 antibody
    • Lipocortin 1 antibody
    • Lipocortin I antibody
    • Lipocortin1 antibody
    • LipocortinI antibody
    • LPC 1 antibody
    • LPC1 antibody
    • p35 antibody
    • Phospholipase A2 inhibitory protein antibody
    see all

Anti-Annexin A1 antibody images



  • Predicted band size : 39 kDa
    Western blot analysis of HepG2 cell lysate labeling Annexin A1 with ab33061 at 1.25µg/ml.
  • Ab33061, at 4 µg/ml, staining Annexin A1 in paraffin embedded human intestine tissue sections by Immuonhistochemistry. The arrows indicate the positively labelled cells (epithelial cells of intestinal glands). Magnification: 400X.Ab33061, at 4 µg/ml, staining Annexin A1 in paraffin embedded human intestine tissue sections by Immuonhistochemistry. The arrows indicate the positively labelled cells (epithelial cells of intestinal glands). Magnification: 400X.
  • All lanes : Anti-Annexin A1 antibody (ab33061) at 2.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 39 kDa
  • Lane 1 : Anti-Annexin A1 antibody (ab33061) at 5 µg/ml
    Lane 2 : Anti-Annexin A1 antibody (ab33061) at 2.5 µg/ml
    Lane 3 : Anti-Annexin A1 antibody (ab33061) at 1.25 µg/ml

    Lane 1 : Cell lysate prepared from human HepG2 cells
    Lane 2 : Cell lysate prepared from human HepG2 cells
    Lane 3 : Cell lysate prepared from human HepG2 cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 39 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Ab33061 recognizes the tagged Annexin A1 protein which has an expected molecular weight of 68 kDa.

References for Anti-Annexin A1 antibody (ab33061)

This product has been referenced in:
  • Wu Y  et al. Evaluation of CRRT effects on pyemic secondary AKI by serum cartilage glycoprotein 39 and Annexin A1. Exp Ther Med 12:2997-3001 (2016). WB ; Human . Read more (PubMed: 27882106) »
  • Gao Y  et al. Differential expression of ANXA1 in benign human gastrointestinal tissues and cancers. BMC Cancer 14:520 (2014). WB . Read more (PubMed: 25038797) »

See all 9 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab33061.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up