• Product name
  • Description
    Goat polyclonal to Annexin A1
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 324-337 of Human Annexin A1 according to NP_000691.1

  • Positive control
    • A431 lysate Rat spleen



Our Abpromise guarantee covers the use of ab81528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 39 kDa.

Approx 37kDa band observed in lysates of cell lines K562 and NIH3T3 (calculated MW of 38.7kDa according to Human NP_000691.1 and Mouse NP_034860.2). Approx 37kDa band observed in Rat and Pig Spleen lysates (calculated MW of 38.7kDa according to Pig NP_001157470.1).


  • Function
    Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
  • Cellular localization
    Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin 1 antibody
    • Annexin A1 antibody
    • Annexin I (lipocortin I) antibody
    • Annexin I antibody
    • Annexin-1 antibody
    • AnnexinA1 antibody
    • AnnexinI antibody
    • ANX 1 antibody
    • ANX A1 antibody
    • ANX1 antibody
    • ANXA 1 antibody
    • ANXA1 antibody
    • ANXA1 protein antibody
    • ANXA1_HUMAN antibody
    • Calpactin 2 antibody
    • Calpactin II antibody
    • Calpactin-2 antibody
    • CalpactinII antibody
    • Chromobindin 9 antibody
    • Chromobindin-9 antibody
    • Chromobindin9 antibody
    • HGNC:533 antibody
    • Lipocortin 1 antibody
    • Lipocortin I antibody
    • Lipocortin1 antibody
    • LipocortinI antibody
    • LPC 1 antibody
    • LPC1 antibody
    • p35 antibody
    • Phospholipase A2 inhibitory protein antibody
    see all

Anti-Annexin A1 antibody images

  • All lanes : Anti-Annexin A1 antibody (ab81528) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : NIH3T3 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 1 µg/ml + Pig spleen lysate at 35 µg

    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 1 µg/ml (for 1 hour) + Rat spleen lysate in RIPA buffer at 35 µg

    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 0.5 µg/ml (for 1 hour) + A431 lysate in RIPA buffer at 35 µg

    Predicted band size : 39 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-Annexin A1 antibody (ab81528)

ab81528 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Pig Tissue lysate - other (protein lysate from lymph node)
Loading amount
30 µg
protein lysate from lymph node
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Angela Moreno

Verified customer

Submitted Mar 02 2011


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