Anti-Annexin-11/ANXA11 antibody (ab166846)
Key features and details
- Mouse polyclonal to Annexin-11/ANXA11
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Annexin-11/ANXA11 antibody
See all Annexin-11/ANXA11 primary antibodies -
Description
Mouse polyclonal to Annexin-11/ANXA11 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Annexin-11/ANXA11 aa 1-550. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P50995 -
Positive control
- Human pancreas tissue lysate; Annexin-11/ANXA11 transfected 293T cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Binds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Cellular localization
Cytoplasm. Melanosome. Nucleus envelope. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Found throughout the nucleoplasm at interphase and during mitosis concentrates around the mitotic apparatus (By similarity). Elevation of intracellular calcium causes relocalization from the nucleoplasm to the nuclear envelope, with little effect on the cytoplasmic pool. Localization to the nuclear envelope is cell-cycle dependent. - Information by UniProt
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Database links
- Entrez Gene: 311 Human
- Omim: 602572 Human
- SwissProt: P50995 Human
- Unigene: 530291 Human
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Alternative names
- 56 kDa autoantigen antibody
- Annexin A11 antibody
- Annexin XI antibody
see all
Images
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Immunofluorescent analysis of HeLa cells, labeling Annexin-11/ANXA11 with ab166846 at 10µg/ml.
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Anti-Annexin-11/ANXA11 antibody (ab166846) at 1 µg/ml + Human Pancreas tissue lysate at 50 µg
Secondary
Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 54 kDa -
All lanes : Anti-Annexin-11/ANXA11 antibody (ab166846) at 1 µg/ml
Lane 1 : Annexin-11/ANXA11 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 54 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab166846 has not yet been referenced specifically in any publications.