Overview

  • Product nameAnti-Annexin A11 antibody
    See all Annexin A11 primary antibodies
  • Description
    Goat polyclonal to Annexin A11
  • SpecificityThis antibody is expected to recognise all three human isoforms.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: YPGYPPPPGGYPP, corresponding to N terminal amino acids 3-15 of Annexin A11 from N Terminus of the protein sequence according to NP_001148; NP_665875; NP_665876

  • Positive control
    • HeLa lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab4121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWestern Blot: approx 55 kDa band seen in HeLa lysate (Predicted MW of approx. 58 kDa according to NP_001148).
    Recommended for use at 0.2-1.0µg/ml.
  • Target

    • FunctionBinds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis.
    • Sequence similaritiesBelongs to the annexin family.
      Contains 4 annexin repeats.
    • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
    • Cellular localizationCytoplasm. Melanosome. Nucleus envelope. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Found throughout the nucleoplasm at interphase and during mitosis concentrates around the mitotic apparatus (By similarity). Elevation of intracellular calcium causes relocalization from the nucleoplasm to the nuclear envelope, with little effect on the cytoplasmic pool. Localization to the nuclear envelope is cell-cycle dependent.
    • Information by UniProt
    • Database links
    • Alternative names
      • 56 kDa autoantigen antibody
      • Annexin A11 antibody
      • Annexin XI antibody
      • Annexin-11 antibody
      • AnnexinA11 antibody
      • AnnexinXI antibody
      • ANX 11 antibody
      • ANX A11 antibody
      • ANX11 antibody
      • ANX11_HUMAN antibody
      • ANXA 11 antibody
      • ANXA11 antibody
      • Autoantigen 56 kD antibody
      • Calcyclin associated annexin 50 antibody
      • Calcyclin-associated annexin 50 antibody
      • CAP 50 antibody
      • CAP-50 antibody
      • CAP50 antibody
      • OTTHUMP00000059806 antibody
      see all

    Anti-Annexin A11 antibody images

    • All lanes : Anti-Annexin A11 antibody (ab4121) at 0.01 µg/ml

      Lane 1 : HeLa lysates (in RIPA buffer)
      Lane 2 : Jurkat lysates (in RIPA buffer)

      Lysates/proteins at 35 µg per lane.


      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

      Primary incubation was 1 hour.

      Detected by chemiluminescence.

    References for Anti-Annexin A11 antibody (ab4121)

    ab4121 has not yet been referenced specifically in any publications.

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