Overview

  • Product name
  • Description
    Rabbit polyclonal to Annexin A13
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Chicken, Guinea pig, Cat, Dog
  • Immunogen

    A region within synthetic peptide: IIEILSGRTS DERQQIKQKY KATYGKELEE VLKSELSGNF EKTALALLDR, corresponding to N terminal amino acids 37-86 of Human Annexin A13

  • Positive control
    • Jurkat cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Tissue specificity
    Gut specific.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Cellular localization
    Cell membrane. Associated with the plasma membrane of undifferentiated, proliferating crypt epithelial cells as well as differentiated villus enterocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin A13 antibody
    • Annexin XIII antibody
    • Annexin, intestine-specific antibody
    • Annexin-13 antibody
    • ANX 13 antibody
    • ANX A13 antibody
    • ANX13 antibody
    • ANX13_HUMAN antibody
    • ANXA13 antibody
    • Intestine Specific Annexin antibody
    • Intestine-specific annexin antibody
    • ISA antibody
    • MGC150460 antibody
    see all

Images

  • Anti-Annexin A13 antibody (ab33066) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Béaslas O  et al. Transcriptome response of enterocytes to dietary lipids: impact on cell architecture, signaling, and metabolism genes. Am J Physiol Gastrointest Liver Physiol 295:G942-52 (2008). WB ; Human . Read more (PubMed: 18755805) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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