Overview

  • Product nameAnti-Annexin A2 antibody
    See all Annexin A2 primary antibodies
  • Description
    Rabbit polyclonal to Annexin A2
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, African Green Monkey
    Predicted to work with: Rat, Sheep, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Annexin II.

    (Peptide available as ab41802.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), HEK293 (Human embryonic kidney cell line), HepG2 (Human hepatocellular liver carcinoma cell line), MCF7 (Human breast adenocarcinoma cell line), and SHSY-5Y (Human neuroblastoma cell line).

Properties

Applications

Our Abpromise guarantee covers the use of ab41803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin A2 peptide (ab41802).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionCalcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Anti-Annexin A2 antibody images

  • All lanes : Anti-Annexin A2 antibody (ab41803) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • IHC image of Annexin II staining in FFPE human breast carcinoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41803, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab41803 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41803, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa cells at 1µg/ml.
  • ICC/IF image of ab41803 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41803, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Annexin A2 antibody (ab41803)

This product has been referenced in:
  • Gonzalez E  et al. Human mammospheres secrete hormone-regulated active extracellular vesicles. PLoS One 9:e83955 (2014). Read more (PubMed: 24404144) »
  • Nuss JE  et al. Multi-Faceted Proteomic Characterization of Host Protein Complement of Rift Valley Fever Virus Virions and Identification of Specific Heat Shock Proteins, Including HSP90, as Important Viral Host Factors. PLoS One 9:e93483 (2014). WB . Read more (PubMed: 24809507) »

See all 13 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (hindpaw skin)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification hindpaw skin
Blocking step LiCOR blocking buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
Username

Dr. maral tajerian

Verified customer

Submitted Aug 26 2015

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (4-20% tris-Glycin gel)
Sample Human Cell lysate - whole cell (Platelet Activating Factor cancer cell lines)
Specification Platelet Activating Factor cancer cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 17 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Sample Monkey Cell (COS)
Specification COS
Permeabilization Yes - 0.1% Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 02 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Sample Human Cell (HUVEC)
Specification HUVEC
Permeabilization Yes - 0.1% Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Human Cell (HUVEC)
Specification HUVEC
Permeabilization Yes - 0.1% Triton X100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 17 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Monkey Cell (cos-7)
Specification cos-7
Permeabilization Yes - 0.1% Triton X100
Fixative Paraformaldehyde
Username

Mrs. ice cool

Verified customer

Submitted Sep 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 22 2012

Thank you for your inquiry.

Unfortunately, we did not experimentally and specifically test if the monomer or tetramer form of Annexin A2 are recognized by these antibodies.

Heterotetramer containing 2 light chains of S100A10/p11 and...

Read More

Thank you for contacting Abcam.

Forthese antibodies I would recommend using reducing conditions and also initially using a 1/1000 primary antibody dilution, although you may to alter this concentration for subsequent western blots.

...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Osteoblast cell line)
Loading amount 10 µg
Specification Osteoblast cell line
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Username

Dr. Rodrigo Alves

Verified customer

Submitted Aug 18 2010

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"