The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/50 - 1/100. Predicted molecular weight: 38 kDa.
FunctionCalcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
Sequence similaritiesBelongs to the annexin family. Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
Western blot - Annexin II antibody - C-terminal (ab75933)
Anti-Annexin A2 antibody - C-terminal (ab75933) at 1/50 dilution + A2058 cell lysate at 35 µg
Predicted band size : 38 kDa Observed band size : 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Annexin II antibody - C-terminal(ab75933)
ab75933 at 1/50 dilution staining Annexin II - C-terminal in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-Annexin A2 antibody - C-terminal (ab75933)