Overview

  • Product name
    Anti-Annexin A2 antibody - N-terminal
    See all Annexin A2 primary antibodies
  • Description
    Rabbit polyclonal to Annexin A2 - N-terminal
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH-conjugated synthetic peptide selected from the N-terminal region of Human Annexin II

  • Positive control
    • Mouse lung tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab75932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 38 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Images

  • Anti-Annexin A2 antibody - N-terminal (ab75932) at 1/50 dilution + mouse lung lysate at 35 µg

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 45 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • ab75932 at 1/50 dilution staining Annexin II - N-terminal in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

  • ICC/IF image of ab75932 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75932, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Ashraf S  et al. Clumping factor A of Staphylococcus aureus interacts with AnnexinA2 on mammary epithelial cells. Sci Rep 7:40608 (2017). Read more (PubMed: 28102235) »
  • Liu X  et al. Proteomic profile in glomeruli of type-2 diabetic KKAy mice using 2-dimensional differential gel electrophoresis. Med Sci Monit 20:2705-13 (2014). Read more (PubMed: 25515740) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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