Annexin A2 overexpression 293T lysate (whole cell) (ab94080)

Overview

  • Product name
    Annexin A2 overexpression 293T lysate (whole cell)
  • Description
    Annexin A2 overexpression lysate
  • General notes
    ab94080 is a 293T cell transfected lysate in which Human Annexin II has been transiently over-expressed using a pCMV-Annexin II plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid. Function: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. PTM: Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated. Similarity: Belongs to the annexin family. Contains 4 annexin repeats.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94080 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Annexin A2 antibody (ab54771) at 1/500 dilution

    Lane 1 : Annexin II 293T Transfected Lysate - Positive Control (ab94080)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

References

ab94080 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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