Overview

  • Product name
  • Description
    Rabbit polyclonal to Annexin A3
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IF, IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse
  • Immunogen

    A region within synthetic peptide: MASIWVGHRG TVRDYPDFSP SVDAEAIQKA IRGIGTDEKM LISILTERSN, corresponding to N terminal amino acids 1-50 of Human Annexin A3

  • Positive control
    • WB: Placenta lysate. IHC-P: Human spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab33068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/100 for 1 hr incubation on mouse cartilage (see Abreview for further information).
IHC - Wholemount Use at an assay dependent concentration.

Target

  • Function
    Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-cyclic phosphate 2-phosphohydrolase antibody
    • 35-alpha calcimedin antibody
    • Annexin A3 antibody
    • Annexin III antibody
    • Annexin-3 antibody
    • ANX3 antibody
    • ANXA 3 antibody
    • Anxa3 antibody
    • ANXA3_HUMAN antibody
    • Calcimedin 35 alpha antibody
    • Inositol 1 2 cyclic phosphate 2 phosphohydrolase antibody
    • Inositol 1 antibody
    • Lipocortin III antibody
    • OTTHUMP00000160701 antibody
    • OTTHUMP00000219237 antibody
    • OTTHUMP00000219238 antibody
    • PAP III antibody
    • PAP-III antibody
    • Placental anticoagulant protein III antibody
    see all

Images

  • Anti-Annexin A3 antibody (ab33068) at 1.25 µg/ml + placenta lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Annexin A3 with ab33068 at 4µg/ml. Positively labelled cells indicated by arrows. Magnification: 400X.

References

This product has been referenced in:
  • Qu S  et al. Protein expression of nucleophosmin, annexin A3 and nm23-H1 correlates with human nasopharyngeal carcinoma radioresistance in vivo. Oncol Lett 12:615-620 (2016). IHC-P ; Human . Read more (PubMed: 27347189) »
  • Yu J  et al. High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers. Oncotarget 7:75279-75292 (2016). WB, IHC-P ; Human . Read more (PubMed: 27661117) »

See all 5 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (placenta)
Total protein in input
3600 µg
Immuno-precipitation step
Protein A
Specification
placenta
Username

Abcam user community

Verified customer

Submitted Oct 02 2015

Application
IHC - Wholemount
Sample
Mouse Embryo (Whole mouse embryo)
Specification
Whole mouse embryo
Username

Abcam user community

Verified customer

Submitted Feb 24 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse (cartilage)
Specification
cartilage
Fixative
Methanol
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5
Username

Abcam user community

Verified customer

Submitted Jul 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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