Overview

  • Product nameAnti-Annexin IV antibody
    See all Annexin IV primary antibodies
  • Description
    Rabbit polyclonal to Annexin IV
  • Tested applicationsSuitable for: IHC-Fr, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within Synthetic peptide: GLGTDEDAII SVLAYRNTAQ RQEIRTAYKS TIGRDLIDDL KSELSGNFEQ , corresponding to N terminal amino acids 31-80 of Human Annexin IV

  • Positive control
    • WB: Placental lysate. IHC-P: Human intestine.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab33009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
ELISA 1/1.5625e+006.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Information by UniProt
  • Database links
  • Alternative names
    • 35 beta calcimedin antibody
    • 35-beta calcimedin antibody
    • AIV antibody
    • Annexin 4 antibody
    • Annexin A4 antibody
    • Annexin IV (placental anticoagulant protein II) antibody
    • Annexin IV antibody
    • Annexin IV placental anticoagulant protein II antibody
    • Annexin-4 antibody
    • AnnexinA4 antibody
    • AnnexinIV antibody
    • ANX 4 antibody
    • ANX A4 antibody
    • ANX4 antibody
    • ANXA 4 antibody
    • ANXA4 antibody
    • ANXA4 protein antibody
    • ANXA4_HUMAN antibody
    • Carbohydrate binding protein P33/P41 antibody
    • Carbohydrate-binding protein p33/p41 antibody
    • Chromobindin 4 antibody
    • Chromobindin-4 antibody
    • Chromobindin4 antibody
    • DKFZp686H02120 antibody
    • Endonexin I antibody
    • EndonexinI antibody
    • Lipocortin IV antibody
    • LipocortinIV antibody
    • MGC75105 antibody
    • OTTHUMP00000160052 antibody
    • OTTHUMP00000202207 antibody
    • P32.5 antibody
    • P33/41 antibody
    • PAP II antibody
    • PAP-II antibody
    • PAPII antibody
    • PIG 28 antibody
    • PIG28 antibody
    • Placental anticoagulant protein II antibody
    • PP4 X antibody
    • PP4-X antibody
    • PP4X antibody
    • Proliferation inducing gene 28 antibody
    • Proliferation inducing protein 28 antibody
    • Protein II antibody
    • Xanx-4 antibody
    • ZAP 36 antibody
    • ZAP36 antibody
    see all

Anti-Annexin IV antibody images

  • Anti-Annexin IV antibody (ab33009) at 1.25 µg/ml + Placental lysate

    Secondary
    HRP conjugated anti Rabbit IgG 1/50000 - 1/100000

    Predicted band size : 36 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelled at Annexin IV with ab33009 at 4-8µg/ml. Positively labelled cells (indicated by arrows) are epithelial cells of intestinal villi. Magnification: 400X.

References for Anti-Annexin IV antibody (ab33009)

This product has been referenced in:
  • Nuss JE  et al. Multi-Faceted Proteomic Characterization of Host Protein Complement of Rift Valley Fever Virus Virions and Identification of Specific Heat Shock Proteins, Including HSP90, as Important Viral Host Factors. PLoS One 9:e93483 (2014). WB . Read more (PubMed: 24809507) »
  • Matsuo Y  et al. Clinicopathological heterogeneity in ovarian clear cell adenocarcinoma: a study on individual therapy practice. Med Mol Morphol N/A:N/A (2014). Read more (PubMed: 25398420) »

See all 4 Publications for this product

Product Wall

Sorry for the delay in getting back to you.

I can now provide further advice for the customer. Whilst the antibody anti-Annexin IV (ab33009) will not be able to detect the fusion construct the customer has been expressing (with ANXA4 reidues ...

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Thank you very much for this information and I am sorry for the delay in getting back to you. I have been away from the office.

With this information provided, I now think I know why the experiment has not been working for the customer. The a...

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Thank you very much for sharing that information with me. It has been very helpful.

In order to ascertain if the antibody Anti-Annexin IV antibody (ab33009) is likely to detect the protein you have been expressing, could you please let me kno...

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Thank you for providing that information.

In order to understand what might be expected could you please answer the following:

1. Was the plasmid constructed by yourself?

2. What size protein was obtained following purific...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibodyhas not been providing you with the expected results.

The details provided will enable us to investigate this case and will provide us with vital i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Small intestinal epithelial cells)
Specification Small intestinal epithelial cells
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Abeer Hammoudi

Verified customer

Submitted May 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (small intestine -epithelial cells extract)
Loading amount 20 µg
Specification small intestine -epithelial cells extract
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Abeer Hammoudi

Verified customer

Submitted May 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (cartilage)
Specification cartilage
Fixative Methanol
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5
Username

Abcam user community

Verified customer

Submitted Aug 08 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"