Overview

  • Product nameAnti-Annexin V antibody
    See all Annexin V primary antibodies
  • Description
    Rabbit polyclonal to Annexin V
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-FoFr, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Annexin V.
    (Peptide available as ab52111)

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab14196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 - 4 µg/ml. Can be blocked with Annexin V Blocking peptide (ab52111).
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20086251

Target

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Involvement in diseasePregnancy loss, recurrent, 3
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anchorin CII antibody
    • Annexin 5 antibody
    • Annexin A5 antibody
    • Annexin V antibody
    • Annexin-5 antibody
    • ANX A5 antibody
    • ANX5 antibody
    • ANXA5 antibody
    • ANXA5_HUMAN antibody
    • Calphobindin I antibody
    • CBP-I antibody
    • Endonexin II antibody
    • ENX2 antibody
    • Lipocortin V antibody
    • PAP I antibody
    • PAP-I antibody
    • Placental anticoagulant protein 4 antibody
    • Placental anticoagulant protein I antibody
    • PLACENTAL PROTEIN 4 antibody
    • Pp4 antibody
    • Thromboplastin inhibitor antibody
    • VAC-alpha antibody
    • Vascular anticoagulant-alpha antibody
    see all

Anti-Annexin V antibody images

  • ab14196 staining Annexin V in mouse embryonic 15 day brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, ermeablized with 0.5% Triton X-100 in PBS, blocked with 10% serum for 1 hour at 25°C and antigen retrieval was by heat mediation in citrate buffer, pH 6. The sample was incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit polyclonal (1/700) was used as the secondary antibody.

    See Abreview

  • Anti-Annexin V antibody (ab14196) + HeLa cell lysate
  • ab14196 staining cells from a mouse organ of Corti (whole mount) by ICC/IF.  The tissue was PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% goat serum, 2% BSA for 1 hour at 25°C.  The primary antibody was diluted 1/200 and incubated with the sample for 2 hours at 25°C.  A Cy3® conjugated sheep anti-rabbit IgG antibody, diluted 1/200 was used as the secondary. Alexa Fluor 488® - Phalloidin was used to label the actin cytoskeleton and DAPI was used to label nuclei.

    See Abreview

  • ab14196 staining cells from a human pancreatic cell line by ICC/IF.  Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C.  The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 22°C.  An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary.

    See Abreview

  • IHC image of Annexin V staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14196, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Annexin V antibody (ab14196)

This product has been referenced in:
  • Crabbé A  et al. Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. PLoS One 10:e0126846 (2015). IHC-P ; Mouse . Read more (PubMed: 25962111) »
  • Tome ME  et al. Identification of P-glycoprotein co-fractionating proteins and specific binding partners in rat brain microvessels. J Neurochem 134:200-10 (2015). Read more (PubMed: 25832806) »

See all 21 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Jaculus jaculus Tissue sections (Skin)
Permeabilization Yes - 0.1% Triton
Specification Skin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Mai Tran

Verified customer

Submitted Nov 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (huamn)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Treatment alpha synuclein
Specification huamn
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. sehee oh

Verified customer

Submitted Jul 13 2016

Application Western blot
Sample Human Cell lysate - whole cell (HBMEC (Human Brain Microvascular Endothelial Cell))
Gel Running Conditions Reduced Denaturing (10)
Loading amount 20 µg
Specification HBMEC (Human Brain Microvascular Endothelial Cell)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 31 2015

Application Western blot
Sample Rat Cell lysate - whole cell (Pericytes)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification Pericytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (bEnd.3)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 20 µg
Specification bEnd.3
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 07 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Sample Mouse Tissue sections (Embryonic 15 day brain)
Specification Embryonic 15 day brain
Permeabilization Yes - 0.5% Triton X-100 in PBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 23 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (lumbar spinal cord, 25 micron)
Specification lumbar spinal cord, 25 micron
Permeabilization Yes - 0.5% Triton X100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 04 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Human Tissue sections (human colon - muscle cells)
Specification human colon - muscle cells
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 26 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Melanoma B16F10 tumor)
Specification Melanoma B16F10 tumor
Fixative 4% Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: autoclaved for 10min at 121 Celcius (10mM Sodium Citrate, pH 6.0)
Permeabilization Yes - 0.1% SDS
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

Submitted May 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Melanoma B16F10 tumor)
Loading amount 30 µg
Specification Melanoma B16F10 tumor
Treatment 5gy Irradiation
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

Submitted Mar 15 2013

1-10 of 21 Abreviews or Q&A

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