- Datasheet
- Specific References (4)
- Protocols
Overview
-
Product nameAnti-Annexin V antibody
See all Annexin V primary antibodies -
DescriptionMouse monoclonal to Annexin V
-
Tested applicationsSuitable for: IP, WB, ICC/IF, IHC-P, Flow Cytmore details
-
Species reactivityReacts with: Human
-
Immunogen
Recombinant full length protein, corresponding to amino acids 1-321 of Human Annexin V
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferpH: 7.20
Constituent: PBS -
Concentration information loading... -
PurityProtein G purified
-
ClonalityMonoclonal
-
IsotypeIgG1
-
Light chain typekappa
-
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Immunohistochemistry kits
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab54775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IHC-P | Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
-
Involvement in diseasePregnancy loss, recurrent, 3
-
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats. -
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
-
Database links
- Entrez Gene: 308 Human
- Omim: 131230 Human
- SwissProt: P08758 Human
- Unigene: 480653 Human
-
Alternative names
- Anchorin CII antibody
- Annexin 5 antibody
- Annexin A5 antibody
see all
Anti-Annexin V antibody images
-
Western blot - Anti-Annexin V antibody (ab54775)
Predicted band size : 36 kDa
ab54775 Western Blot analysis of ANXA5 expression in Hela cells. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V antibody (ab54775)Annexin V antibody (ab54775) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon. -
Immunocytochemistry/ Immunofluorescence - Anti-Annexin V antibody (ab54775)ab54775 at 10 ug/ml staining Annexin V in human Hela cells by Immunocytochemistry / Immunofluorescence. -
Flow Cytometry - Anti-Annexin V antibody (ab54775)Overlay histogram showing HeLa cells stained with ab54775 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54775, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. -
Immunoprecipitation - Anti-Annexin V antibody (ab54775)Immunoprecipitation of ANXA5 transfected lysate using ab54775 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with a ANXA5 rabbit polyclonal antibody. -
Western blot - Anti-Annexin V antibody (ab54775)
Predicted band size : 36 kDa
Western Blot analysis of ANXA5 expression in transfected 293T cell line by ab54775.
Lane 1: ANXA5 transfected lysate(35.9 KDa).
Lane 2: Non-transfected lysate. -
Immunocytochemistry/ Immunofluorescence - Anti-Annexin V antibody (ab54775)ab54775 staining annexin V in HeLa cells treated with pentoxifylline (ab120725), by ICC/IF. Increase of annexin V correlates with increased concentration of pentoxifylline, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120725 (pentoxifylline) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab54775 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
References for Anti-Annexin V antibody (ab54775)
This product has been referenced in:
- Liao J et al. Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells. Nat Genet 47:469-78 (2015). ICC/IF ; Human . Read more (PubMed: 25822089) »
- Huang P et al. P2X4 Forms Functional ATP-activated Cation Channels on Lysosomal Membranes Regulated by Luminal pH. J Biol Chem 289:17658-67 (2014). WB ; Human . Read more (PubMed: 24817123) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"