- Datasheet
- Specific References (5)
- Protocols
Overview
-
Product nameAnti-Annexin V antibody
See all Annexin V primary antibodies -
DescriptionMouse monoclonal to Annexin V
-
Host speciesMouse
-
Tested applicationsSuitable for: IP, WB, ICC/IF, IHC-P, Flow Cytmore details
-
Species reactivityReacts with: Human
-
Immunogen
Recombinant full length protein, corresponding to amino acids 1-321 of Human Annexin V
-
General notes
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab108194 as a replacement.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferpH: 7.20
Constituent: PBS -
Concentration information loading... -
PurityProtein G purified
-
ClonalityMonoclonal
-
IsotypeIgG1
-
Light chain typekappa
-
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Immunohistochemistry kits
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab54775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IHC-P | Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
-
Involvement in diseasePregnancy loss, recurrent, 3
-
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats. -
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
-
Database links
- Entrez Gene: 308 Human
- Omim: 131230 Human
- SwissProt: P08758 Human
- Unigene: 480653 Human
-
Alternative names
- Anchorin CII antibody
- Annexin 5 antibody
- Annexin A5 antibody
see all
Images
-
Western blot - Anti-Annexin V antibody (ab54775)ab54775 Western Blot analysis of ANXA5 expression in Hela cells. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V antibody (ab54775)Annexin V antibody (ab54775) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon. -
Immunocytochemistry/ Immunofluorescence - Anti-Annexin V antibody (ab54775)ab54775 at 10 ug/ml staining Annexin V in human Hela cells by Immunocytochemistry / Immunofluorescence. -
Flow Cytometry - Anti-Annexin V antibody (ab54775)Overlay histogram showing HeLa cells stained with ab54775 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54775, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. -
Immunoprecipitation - Anti-Annexin V antibody (ab54775)Immunoprecipitation of ANXA5 transfected lysate using ab54775 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with a ANXA5 rabbit polyclonal antibody. -
Western blot - Anti-Annexin V antibody (ab54775)Western Blot analysis of ANXA5 expression in transfected 293T cell line by ab54775.
Lane 1: ANXA5 transfected lysate(35.9 KDa).
Lane 2: Non-transfected lysate. -
Immunocytochemistry/ Immunofluorescence - Anti-Annexin V antibody (ab54775)ab54775 staining annexin V in HeLa cells treated with pentoxifylline (ab120725), by ICC/IF. Increase of annexin V correlates with increased concentration of pentoxifylline, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120725 (pentoxifylline) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab54775 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
References
This product has been referenced in:
- Liao J et al. Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells. Nat Genet 47:469-78 (2015). ICC/IF ; Human . Read more (PubMed: 25822089) »
- Arroyo-Berdugo Y et al. Involvement of ANXA5 and ILKAP in susceptibility to malignant melanoma. PLoS One 9:e95522 (2014). WB . Read more (PubMed: 24743186) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"