The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at an assay dependent dilution.
I-ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 38 kDa.
Array: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Involvement in diseasePregnancy loss, recurrent, 3
Sequence similaritiesBelongs to the annexin family. Contains 4 annexin repeats.
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.