Validated using a knockout cell line
Recombinant
RabMAb

Anti-Annexin V antibody [EPR3979] (ab108321)

Overview

  • Product name
    Anti-Annexin V antibody [EPR3979]
    See all Annexin V primary antibodies
  • Description
    Rabbit monoclonal [EPR3979] to Annexin V
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Annexin V aa 1-100. The exact sequence is proprietary.

  • Positive control
    • HeLa, JAR, and fetal kidney lysates; Human gastric tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
    • Involvement in disease
      Pregnancy loss, recurrent, 3
    • Sequence similarities
      Belongs to the annexin family.
      Contains 4 annexin repeats.
    • Domain
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
      A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • Post-translational
      modifications
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • Anchorin CII antibody
      • Annexin 5 antibody
      • Annexin A5 antibody
      • Annexin V antibody
      • Annexin-5 antibody
      • ANX A5 antibody
      • ANX5 antibody
      • ANXA5 antibody
      • ANXA5_HUMAN antibody
      • Calphobindin I antibody
      • CBP-I antibody
      • Endonexin II antibody
      • ENX2 antibody
      • Lipocortin V antibody
      • PAP I antibody
      • PAP-I antibody
      • Placental anticoagulant protein 4 antibody
      • Placental anticoagulant protein I antibody
      • PLACENTAL PROTEIN 4 antibody
      • Pp4 antibody
      • Thromboplastin inhibitor antibody
      • VAC-alpha antibody
      • Vascular anticoagulant-alpha antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Annexin V knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab108321 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab108321 was shown to specifically react with Annexin V in wild-type cells as signal was lost in Annexin V knockout cells. Wild-type and Annexin V knockout samples were subjected to SDS-PAGE. Ab108321 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Annexin V antibody [EPR3979] (ab108321) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 36 kDa

    • Immunohistochemical analysis of Annexin V in paraffin embedded Human gastric tissue, using ab108321 at a 1/100 dilution.

    References

    This product has been referenced in:
    • Campoy I  et al. Exosome-like vesicles in uterine aspirates: a comparison of ultracentrifugation-based isolation protocols. J Transl Med 14:180 (2016). Read more (PubMed: 27317346) »
    • Liu B  et al. Altered protein expression in gestational diabetes mellitus placentas provides insight into insulin resistance and coagulation/fibrinolysis pathways. PLoS One 7:e44701 (2012). WB, IHC-P ; Human . Read more (PubMed: 22970290) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
    Sample
    Human Tissue sections (human colon - muscle cells)
    Specification
    human colon - muscle cells
    Permeabilization
    Yes - 0.2% Triton X-100 and PBS
    Fixative
    Formaldehyde
    Username

    Mrs. Maria Cecilia Ybanez

    Verified customer

    Submitted Mar 11 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% T ween 20, pH 6.0)
    Sample
    Human Tissue sections (human colon)
    Specification
    human colon
    Permeabilization
    Yes - 0.2% Triton X-100 and PBS
    Fixative
    Formaldehyde
    Username

    Dr. Steffen Rickelt

    Verified customer

    Submitted Nov 06 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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