1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Involvement in diseasePregnancy loss, recurrent, 3
Sequence similaritiesBelongs to the annexin family. Contains 4 annexin repeats.
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Western blot - Annexin V antibody [EPR3979] (ab108321)
All lanes : Anti-Annexin V antibody [EPR3979] (ab108321) at 1/1000 dilution
Lane 1 : HeLa cell lysate Lane 2 : JAR cell lysate Lane 3 : fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Annexin V antibody [EPR3979] (ab108321)
Immunohistochemical analysis of Annexin V in paraffin embedded Human gastric tissue, using ab108321 at a 1/100 dilution.
References for Anti-Annexin V antibody [EPR3979] (ab108321)
This product has been referenced in:
Liu B et al. Altered protein expression in gestational diabetes mellitus placentas provides insight into insulin resistance and coagulation/fibrinolysis pathways. PLoS One7:e44701 (2012).
Read more (PubMed: 22970290) »