1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Involvement in disease
Pregnancy loss, recurrent, 3
Belongs to the annexin family. Contains 4 annexin repeats.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. A pair of annexin repeats may form one binding site for calcium and phospholipid.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Western blot - Anti-Annexin V antibody [EPR3979] (ab108321)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: Annexin V knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab108321 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108321 was shown to specifically react with Annexin V in wild-type cells as signal was lost in Annexin V knockout cells. Wild-type and Annexin V knockout samples were subjected to SDS-PAGE. Ab108321 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Annexin V antibody [EPR3979] (ab108321)
All lanes : Anti-Annexin V antibody [EPR3979] (ab108321) at 1/1000 dilution
Lane 1 : HeLa cell lysate Lane 2 : JAR cell lysate Lane 3 : fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Annexin V antibody [EPR3979] (ab108321)
Immunohistochemical analysis of Annexin V in paraffin embedded Human gastric tissue, using ab108321 at a 1/100 dilution.
Campoy I et al. Exosome-like vesicles in uterine aspirates: a comparison of ultracentrifugation-based isolation protocols. J Transl Med14:180 (2016).
Read more (PubMed: 27317346) »
Liu B et al. Altered protein expression in gestational diabetes mellitus placentas provides insight into insulin resistance and coagulation/fibrinolysis pathways. PLoS One7:e44701 (2012).
Read more (PubMed: 22970290) »