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Annexin V-Biotin Apoptosis Staining / Detection Kit (ab1419) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -beta-gal, etc.
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|Annexin V-Biotin||1 x 500µl|
|Binding Buffer||1 x 50ml|
|Propidium iodide||1 x 500µl|
Our Abpromise guarantee covers the use of ab14190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent dilution.|