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Abcam's Annexin V-FITC Apoptosis Staining / Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.
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|Annexin V-FITC||1 x 500µl|
|Binding Buffer||1 x 50ml|
|Propidium iodide||1 x 500µl|
Our Abpromise guarantee covers the use of ab14085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent dilution.|
HeLa cells were harvested with trypsinization together with floating non-viable cells. Cells were washed with PBS and suspended in sodium citrate buffer 20 minutes prior to analysis. HeLa cells were treated with Mitoxantrone (MX) and MX +Imatinib for 48 hours. The samples were then stained with Annexin V-FITC Apoptosis Staining/Detection kit (ab14085). A FACSCalibur flow cytometer was used for cell cycle analysis.
PC3 cells were seeded at 106 cells/ml and incubated overnight and then treated with CTA095 at various concentrations for 24hours. Apoptosis was then analyzed using Annexin-V FITC apoptosis detection kit (ab14085).
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