Overview

  • Product nameAnnexin V Human ELISA kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Overall < 10%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.33 ng/ml
  • Range
    0.78 ng/ml - 50 ng/ml
  • Recovery

    85 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Annexin V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Annexin V concentrations in cell culture supernatant and serum.

    Annexin V specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Annexin V detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Annexin V sample captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Information by UniProt
  • Alternative names
    • Anchorin CII
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • Lipocortin V
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Annexin V Human ELISA kit images

  • Representative Standard Curve using ab119503.

Protocols

References for Annexin V Human ELISA kit (ab119503)

ab119503 has not yet been referenced specifically in any publications.

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please do let us know the details of your protocol, the steps you have performed as well as the raw annotated data, andwe will be pleased to look into this in more details.

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Thank you very much for your inquiry.

Once the standard is reconstituted, we recommend using them immediately and then discarding the remaining portion.
We have not performed stability studies regarding the long -term storage of reconsti...

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Once the standard is reconstituted, we recommend using them immediately and then discarding the remaining portion.


We have not performed stability studies regarding the long -term storage of reconstituted standard protein so unfortunate...

Read More

Thank you for contacting us. The reconstitution instructions for the standard are lot specific and will be written on the vial. If for any reason it is not, please let us know your lot number and we will be happy to find that information for you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"