Annexin V overexpression 293T lysate (whole cell) (ab76216)

Overview

  • Product nameAnnexin V overexpression 293T lysate (whole cell)
    See all Annexin V lysates
  • Description
    Annexin V overexpression lysate
  • General notesab76216 is a 293T cell transfected lysate in which Human Annexin V has been transiently over-expressed using a pCMV-Annexin V plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared, click here.
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundDisease: Pregnancy loss, recurrent, 3 Domain: The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. A pair of annexin repeats may form one binding site for calcium and phospholipid. Function: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. PTM: S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. Similarity: Belongs to the annexin family. Contains 4 annexin repeats.

Associated products

Applications

Our Abpromise guarantee covers the use of ab76216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Annexin V overexpression 293T lysate (whole cell) images

  • ab76216 at 15μg/lane on an SDS-PAGE gel.

  • All lanes : Anti-Annexin V antibody (ab54775) at 1/500 dilution

    Lane 1 : Annexin V overexpression 293T lysate (whole cell) (ab76216)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Annexin V overexpression 293T lysate (whole cell) (ab76216)

ab76216 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"