Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)

Overview

  • Product name
    Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit
    See all Annexin V kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Direct
  • Assay time
    0h 10m
  • Product overview

    Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation. Results can be analyzed by flow cytometry or fluorescence microscopy.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applications
    Suitable for: FM, Flow Cytmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab14159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Protocols

References

ab14159 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



We do not have any experimentaldata for theuse of this kitwith GFP, howeverI think this should be ok sincethe kit uses the tandem (PE-Cy5) for which the excitation wavelengthis 488 nm and the emission 670nm. This should not interfere with the...

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I'm very sorry to hear you are having problems with this kit and for the shortages you have experienced. I have arranged for 2 kits to be sent to you as soon as possible (order reference 80894) and apologise for the inconvenience. I also enclose the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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