Overview

  • Product nameAnti-Annexin VI antibody
    See all Annexin VI primary antibodies
  • Description
    Rabbit polyclonal to Annexin VI
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    QGAKYRGSIHDFPGFDPNQ

    , corresponding to amino acids 5-23 of Human Annexin VI.

  • Positive control
    • IHC-P: Human L8 cells. Liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab31026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/50.

1/50 for 30 min at room temperature. Requires boiling of tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target

  • FunctionMay associate with CD21. May regulate the release of Ca(2+) from intracellular stores.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 8 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylated in response to growth factor stimulation.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 67 kDa calelectrin antibody
    • Annexin A6 antibody
    • Annexin VI antibody
    • Annexin VI p68 antibody
    • Annexin-6 antibody
    • AnnexinA6 antibody
    • AnnexinVI antibody
    • ANX 6 antibody
    • ANX A6 antibody
    • ANX6 antibody
    • ANXA 6 antibody
    • ANXA6 antibody
    • ANXA6_HUMAN antibody
    • Calcium binding protein p68 antibody
    • Calelectrin antibody
    • Calphobindin II antibody
    • Calphobindin-II antibody
    • CalphobindinII antibody
    • CBP 68 antibody
    • CBP68 antibody
    • Chromobindin 20 antibody
    • Chromobindin-20 antibody
    • Chromobindin20 antibody
    • CPB II antibody
    • CPB-II antibody
    • CPBII antibody
    • Lipocortin VI antibody
    • LipocortinVI antibody
    • p68 antibody
    • p70 antibody
    • Protein III antibody
    • ProteinIII antibody
    see all

Anti-Annexin VI antibody images

  • Anti-Annexin VI antibody (ab31026) at 1/500 dilution + Mouse heart tissue lysate at 50 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/7500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 76 kDa


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Ab31026, at a dilution of 1/50, staining formalin fixed paraffin embedded Annexin VI in human liver tissue by Immunohistochemistry.
  • All lanes : Anti-Annexin VI antibody (ab31026) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 4 : A498 (Human Kidney Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 76 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • Anti-Annexin VI antibody (ab31026) at 1/25 dilution + lysate prepared from 293 cells

    Predicted band size : 76 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-Annexin VI antibody (ab31026)

This product has been referenced in:
  • Swaggart KA  et al. Annexin A6 modifies muscular dystrophy by mediating sarcolemmal repair. Proc Natl Acad Sci U S A 111:6004-9 (2014). ICC/IF ; Mouse . Read more (PubMed: 24717843) »
  • Garrido-Gómez T  et al. Deciphering the proteomic signature of human endometrial receptivity. Hum Reprod 29:1957-67 (2014). Human . Read more (PubMed: 25106620) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-15% gradient gel)
Sample Rat Cell lysate - nuclear (rat ventricular cardiomyocyte)
Specification rat ventricular cardiomyocyte
Treatment 1. No treatment 2. Endothelin-1 100nM 48hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted Feb 23 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (Heart)
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 26 2013

I am very pleased to hear you would like to accept our offer and test ab31026 in chicken. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for chicken samples and include this...

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Thank you for contacting us. Of our Annexin VI antibodies, ab19416 is the most likely to cross-react with chicken (UniProt: P51901). The homologies of their immunogens with the chicken sequence is as follows: ab115811: 69% ab30126/ ab...

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