• Product name
  • Description
    Rabbit polyclonal to Annexin VI
  • Specificity
    ab52221 detects endogenous levels of total Annexin A6 protein
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human Annexin A6.

  • Positive control
    • Extracts from 293 cells



Our Abpromise guarantee covers the use of ab52221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ELISA 1/10000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 8 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    Phosphorylated in response to growth factor stimulation.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 67 kDa calelectrin antibody
    • Annexin A6 antibody
    • Annexin VI antibody
    • Annexin VI p68 antibody
    • Annexin-6 antibody
    • AnnexinA6 antibody
    • AnnexinVI antibody
    • ANX 6 antibody
    • ANX A6 antibody
    • ANX6 antibody
    • ANXA 6 antibody
    • ANXA6 antibody
    • ANXA6_HUMAN antibody
    • Calcium binding protein p68 antibody
    • Calelectrin antibody
    • Calphobindin II antibody
    • Calphobindin-II antibody
    • CalphobindinII antibody
    • CBP 68 antibody
    • CBP68 antibody
    • Chromobindin 20 antibody
    • Chromobindin-20 antibody
    • Chromobindin20 antibody
    • CPB II antibody
    • CPB-II antibody
    • CPBII antibody
    • Lipocortin VI antibody
    • LipocortinVI antibody
    • p68 antibody
    • p70 antibody
    • Protein III antibody
    • ProteinIII antibody
    see all

Anti-Annexin VI antibody images

  • All lanes : Anti-Annexin VI antibody (ab52221) at 1/500 dilution

    Lane 1 : extracts from 293 cells,
    Lane 2 : extracts from 293 cells, with immunizing peptide.

    Predicted band size : 75 kDa
    Observed band size : 75 kDa
  • IHC image of ab52221 staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52221, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Annexin VI antibody (ab52221) at 1/1000 dilution + Recombinant Human Annexin VI protein (ab92968) at 0.1 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Anti-Annexin VI antibody (ab52221)

This product has been referenced in:
  • Dismuke WM  et al. Mechanism of Fibronectin Binding to Human Trabecular Meshwork Exosomes and Its Modulation by Dexamethasone. PLoS One 11:e0165326 (2016). WB ; Human . Read more (PubMed: 27783649) »
  • Rüetschi U  et al. SILAC-Based Quantitative Proteomic Analysis of Diffuse Large B-Cell Lymphoma Patients. Int J Proteomics 2015:841769 (2015). WB . Read more (PubMed: 26060582) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. Of our Annexin VI antibodies, ab19416 is the most likely to cross-react with chicken (UniProt: P51901). The homologies of their immunogens with the chicken sequence is as follows: ab115811: 69% ab30126/ ab...

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