The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC: Use at an assay dependent dilution with methanol or 1% paraformaldehyde/ 0.1% glutaraldehyde.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 47 or 51 kDa depending on the tissue/cell used (predicted molecular weight: 53 kDa). A degradation product of approximately 30 kDa may also be detected.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Tissue specificityIsoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta.
Sequence similaritiesBelongs to the annexin family. Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.