Anti-Annexin VII antibody [203-217/6] (ab49838)

Overview

  • Product nameAnti-Annexin VII antibody [203-217/6]
    See all Annexin VII primary antibodies
  • Description
    Mouse monoclonal [203-217/6] to Annexin VII
  • Tested applicationsSuitable for: IHC-Fr, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human
  • Immunogen

    Recombinant full length protein (Mouse)

  • Positive control
    • Whole cell extract of culture mouse connective tissue (A9) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab49838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
ICC
WB
  • Application notesICC: Use at an assay dependent dilution with methanol or 1% paraformaldehyde/ 0.1% glutaraldehyde.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 47 or 51 kDa depending on the tissue/cell used (predicted molecular weight: 53 kDa). A degradation product of approximately 30 kDa may also be detected.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Annexin VII antibody [203-217/6] images

    • Anti-Annexin VII antibody [203-217/6] (ab49838) at 1/1000 dilution + whole cell lysate prepared from human oesophageal squamous cell carcinoma PJ41 cells at 20 µg

      Secondary
      Goat anti-mouse IgG conjugated to HRP at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    References for Anti-Annexin VII antibody [203-217/6] (ab49838)

    ab49838 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Oral)
    Loading amount 20 µg
    Specification Oral
    Gel Running Conditions Reduced Non-Denaturing (Native)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

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    Verified customer

    Submitted Jan 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"