- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Annexin VII antibody [203-217/6]
See all Annexin VII primary antibodies -
DescriptionMouse monoclonal [203-217/6] to Annexin VII
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Host speciesMouse
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Tested applicationsSuitable for: IHC-Fr, ICC, WBmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human
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Immunogen
Recombinant full length protein (Mouse)
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Positive control
- Whole cell extract of culture mouse connective tissue (A9) cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4 -
Concentration information loading... -
PurityIgG fraction
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Purification notesPurified Immunoglobulin
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ClonalityMonoclonal
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Clone number203-217/6
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab49838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | ||
| ICC | ||
| WB |
Application notes
ICC: Use at an assay dependent dilution with methanol or 1% paraformaldehyde/ 0.1% glutaraldehyde.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 47 or 51 kDa depending on the tissue/cell used (predicted molecular weight: 53 kDa). A degradation product of approximately 30 kDa may also be detected.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 47 or 51 kDa depending on the tissue/cell used (predicted molecular weight: 53 kDa). A degradation product of approximately 30 kDa may also be detected.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
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Tissue specificityIsoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta.
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Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats. -
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
- Information by UniProt
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Database links
- Entrez Gene: 423747 Chicken
- Entrez Gene: 533360 Cow
- Entrez Gene: 310 Human
- Entrez Gene: 11750 Mouse
- Entrez Gene: 100338267 Rabbit
- Entrez Gene: 155423 Rat
- Omim: 186360 Human
- SwissProt: P20072 Cow
see all -
Alternative names
- Annexin A7 antibody
- Annexin VII antibody
- Annexin-7 antibody
see all
Images
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![Western blot - Anti-Annexin VII antibody [203-217/6] (ab49838)](http://a.abcam.com/ps/products/49/ab49838/Images/Annexin-VII-Primary-antibodies-ab49838-3.jpg)
Western blot - Anti-Annexin VII antibody [203-217/6] (ab49838)Image courtesy of an anonymous Abreview.Anti-Annexin VII antibody [203-217/6] (ab49838) at 1/1000 dilution + whole cell lysate prepared from human oesophageal squamous cell carcinoma PJ41 cells at 20 µg
Secondary
Goat anti-mouse IgG conjugated to HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 47 kDa (why is the actual band size different from the predicted?)
Exposure time: 5 minutes
Blocking step performed using 5% milk for 1 hour at 4°C.
Protocols
References
ab49838 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"