Overview

  • Product name
  • Description
    Mouse polyclonal to Annexin VII
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Annexin VII protein (NP_001147.1).

  • Positive control
    • Human liver tissue lysate; Hela cell lysate; Annexin VII transfected 293T cell lysate; Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
  • Tissue specificity
    Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin A7 antibody
    • Annexin VII antibody
    • Annexin-7 antibody
    • ANX7 antibody
    • ANXA7 antibody
    • ANXA7_HUMAN antibody
    • SNX antibody
    • Synexin antibody
    see all

Images

  • Anti-Annexin VII antibody (ab88539) at 1 µg/ml + human liver tissue lysate at 50 µg

    Predicted band size : 53 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • Anti-Annexin VII antibody (ab88539) at 1 µg/ml + Hela cell lysate at 50 µg

    Predicted band size : 53 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Annexin VII antibody (ab88539) at 1 µg/ml

    Lane 1 : Annexin VII transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • ab88539, at 10 µg/ml, staining Annexin VII in Hela cells by Immunofluorescence.

References

ab88539 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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